Instructions for Purifying Bovine Tuberculin (For non-human primates)

This product is prepared by inoculating bovine Mycobacterium tuberculosis into a suitable culture medium, followed by inactivation, soaking, sterile filtration, and purification.

[ Main ingredient 】 This product is a purified protein derivative of Mycobacterium bovis.

【Characteristics】    The liquid product is a clear, colorless or slightly yellowish-brown liquid; the freeze-dried product is a loose clump that is milky white or slightly pale yellowish-brown and dissolves rapidly upon addition of a diluent.

[Function and Use] For intradermal allergy testing by Bovine Mycobacterium tuberculosis caused by Animal tuberculosis.

【Usage】 ( 1 ) Eyelid Intradermal hypersensitivity test    Liquid purified bovine tuberculin does not need to be diluted; freeze-dried products should be reconstituted first. 5ml Dilute with water for injection (or saline solution) to... 10 Toufen /ml ( 20000IU ) Animals should be injected. Moderate amount of Sedative ， Take monkeys as an example, The eyelid is the preferred site for testing. Specific location is: Near the eyelashes at the edge of the upper eyelid, With 75 Disinfect the injection site with 75% alcohol, and allow the alcohol to evaporate completely before proceeding. Use 1 m l The syringe is used for intradermal injection of tuberculin. ， Each one Animals awaiting inspection Injection 0.1ml , one injection per dose Replace the injection needle once. Needle Specifications Best For 0.45mm) 。

(2) Abdominal skin Intradermal hypersensitivity test    The skin on the abdomen can be used as a donor site. , use 75 % alcohol Perform surface Disinfect, and after the alcohol has completely evaporated, proceed with... 1m l The syringe is used for intradermal injection. Tuberculin ， Each one Animals awaiting inspection Injection 0.1ml , one injection per dose Replace the injection needle once. Needle Specifications Best For 0.45mm) 。

Injection site and Any bruise must be recorded. In 24 h 、48 h and 72 h Observation Eyelid Injection site, Or measure the results of the abdominal skin test (the abdominal skin test requires using a vernier caliper to measure the injection site). Delayed-type hypersensitivity the diameter, calculate the average) And record. Recommended with 48h The result shall prevail.

[ Judging criteria 】

① +++: The swelling is severe, even to the point of complete closure of the eyes, with noticeable canthal involvement. The lesions typically rupture several days later and fail to heal within a week.

② ++: Swelling is prominent, the upper eyelid is reddened, and the two eyes look noticeably different. 72h It gradually subsided afterward.

③ +: The swelling is not obvious, but redness has spread to the entire upper eyelid. 48h Obviously, 72h Post-erasure.

④ ± : No swelling, mild redness at the injection site. 48h Fade away.

⑤ -: Eyelid None Any reaction is the same as in the eye that was not injected.

Whenever it appears “ +” And above Reaction of the Animals awaiting inspection ， Can be immediately determined as positive, Conduct a re-examination. ， The re-examination can be used. gamma- Performed using an in vitro interferon release assay or other methods. Diagnosis 。

Negative and Suspicious Animals awaiting inspection , after injection 96 h and 120 h Observe separately again. 1 Next, in case of individual instances Animals awaiting inspection Late-onset delayed-type hypersensitivity reaction.

Any reaction determined to be suspicious Animals awaiting inspection , at 14-15d After Perform a re-examination. If the results still indicate a suspicious reaction, then... 14-15d Re-examine. ：

① In three trials, one. “ ± or +” , second time “ -” judge “ -”。

② Twice or more “ ± ” , judgment “ ± ”。

③ Once “ -” or “ ± ” , second time “ +” , judgment “ +”。

[ Specifications 】   Liquid products: ( 1 ) 5ml/ Bottle    ( 50 Toufen / Bottle)

Lyophilized product: ( 1 ) 2ml/ Bottle    ( 50 Toufen / Bottle)

[ Storage and Shelf Life 】   Liquid Product Storage conditions are: 2 ~ 8℃ Save; valid until 24 months.

Freeze-drying Product Storage conditions are: 2 ~ 8 Celsius or -15℃ The following is saved and valid until: 120 months.

[ Precautions 】   ( 1 ) Protect from sunlight and high temperatures. 2 ) After dilution, use up within the same day. 3 ) Used Vial 、 Syringes, etc. Utensils and Unused Reagent Disinfection treatment should be carried out.